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The Two-Partner Secretion (TPS) Family [Function: Biogenesis/Secretion] Seed alignment | Full alignment in OMPdb

The type V secretion pathway encompasses the autotransporter proteins (AT), the two-partner secretion system (TPS) and the type AT-2 family of proteins. TPS systems are composed of two separate proteins, with TpsA being the secreted protein and TpsB its specific transporter. The secreted proteins are exported in a Sec-dependent manner across the inner membrane, after which they cross the outer membrane with the help of their cognate transporters. Translocation appears to be folding-sensitive, indicating that TpsA proteins cross the periplasm and the outer membrane in non-native conformations and fold progressively at the cell surface. A major difference of the TPS pathways compared to the AT arises from the manner in which specificity is established between the secreted protein and its transporter. The TPS pathway has solved the question of specific recognition between the TpsA proteins and their transporters by the addition to the TpsA proteins of an N-proximal module, the conserved TPS domain, which represents a hallmark of the TPS pathway. The exoproteins of the TPS have been reported to be adhesins, haem-binding proteins, antigenic factors or haemolysins/cytolysins. The structure of the TPS domain has been solved and shows to fold as a beta helix. The crystal structure of the transmembrane domain of the Bordetella pertussis TpsB transporter (FhaC) has been resolved revealling a ß-barrel with 16 transmembrane strands.

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Literature references

Autotransporter and two-partner secretion: delivery of large-size virulence factors by gram-negative bacterial pathogens
Crit Rev Microbiol. 2004;30(4):275-86. doi: 10.1080/10408410490499872.
PMID: 15646401

Protein secretion through autotransporter and two-partner pathways
Biochim Biophys Acta. 2004 Nov 11;1694(1-3):235-57. doi: 10.1016/j.bbamcr.2004.03.008.
PMID: 15546669

The autotransporter secretion system
Res Microbiol. 2004 Mar;155(2):53-60. doi: 10.1016/j.resmic.2003.10.002.
PMID: 14990256

Structure of the membrane protein FhaC: a member of the Omp85-TpsB transporter superfamily
Science. 2007 Aug 17;317(5840):957-61. doi: 10.1126/science.1143860.
PMID: 17702945

Proteins in this family with 3D-structure



Elixir UTH dib compgen