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The Antimicrobial peptide resistance and lipid A acylation protein (PagP) Family [Function: Enzyme] Seed alignment | Full alignment in OMPdb

This family consists of several bacterial antimicrobial peptide resistance and lipid A acylation (PagP) proteins. In a number of pathogenic Gram-negative bacteria, PagP offers resistance to certain cationic antimicrobial peptides produced during the host innate immune response. PagP is an integral outer membrane enzyme (acyltransferase) that transfers a palmitate chain from a phospholipid to lipid A (endotoxin). PagP can distinguish lipid acyl chains that differ by a single methylene unit, indicating that the enzyme possesses a remarkably precise hydrocarbon ruler. The crystal structure of PagP from Escherichia coli has been solved, suggesting an 8-stranded ß-barrel, preceded by an amphipathic alpha helix, with an interior hydrophobic pocket occupied by a single detergent molecule. Acyl-group specificity is modulated by mutation of Gly(88) lining the bottom of the hydrophobic pocket and therefore confirming the hydrocarbon ruler mechanism for palmitate recognition. Three amino acid residues, critical for enzymatic activity are localized to extracellular loops in order to interact with the polar head groups of lipid A. Hence, the active site of PagP is situated on the outer surface of the outer membrane, similar to the outer membrane phospholipases.


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Literature references

Structural biology of membrane-intrinsic beta-barrel enzymes: sentinels of the bacterial outer membrane
Biochim Biophys Acta. 2008 Sep;1778(9):1881-96. doi: 10.1016/j.bbamem.2007.07.021. Epub 2007 Aug 11.
PMID: 17880914

Solution structure and dynamics of the outer membrane enzyme PagP by NMR
Proc Natl Acad Sci U S A. 2002 Oct 15;99(21):13560-5. doi: 10.1073/pnas.212344499. Epub 2002 Sep 30.
PMID: 12357033

A hydrocarbon ruler measures palmitate in the enzymatic acylation of endotoxin
EMBO J. 2004 Aug 4;23(15):2931-41. doi: 10.1038/sj.emboj.7600320. Epub 2004 Jul 22.
PMID: 15272304

Proteins in this family with 3D-structure

P37001


Elixir UTH dib compgen