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The Outer membrane porin (OprD) family [Function: Specific diffusion channels] Seed alignment | Full alignment | Pfam page | Pfam Wiki page | TC-DB page

The OprD family includes a number of porins in Pseudomonas species and other Gram-negative bacteria that appear to exhibit a variety of substrate specificities. OprD facilitates the diffusion of basic amino acids as well as some structurally analogous ß-lactam antibiotics like imipenem. Mutational inactivation of the oprD gene is associated with carbapenem resistance in Pseudomonas aeruginosa, whereas the C-terminal portion of OprD and, in particular, the hypothetical loop L7, was responsible for the unusual meropenem hypersusceptibility. There is also evidence that OprD porin of Pseudomonas aeruginosa bears protease activity with the catalytic activity established in residues His156, Asp208, and Ser296. However, not all family members have these residues conserved, suggesting that are not all enzymes. Various proteins of the family have shown different specificities in the uptake of metabolites including glycine-glutamate, histidine, proline, tyrosine, cis-aconitate and pyroglutamate. X-ray crystal structure of OprD from Pseudomonas aeruginosa has revealed a monomeric 18-stranded ß-barrel.


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Literature references


Getting Drugs through Small Pores: Exploiting the Porins Pathway in Pseudomonas aeruginosa
ACS Infect Dis. 2018 Oct 12;4(10):1519-1528. doi: 10.1021/acsinfecdis.8b00149. Epub 2018 Aug 8.
PMID: 30039960

Structure, function and regulation of Pseudomonas aeruginosa porins
FEMS Microbiol Rev. 2017 Sep 1;41(5):698-722. doi: 10.1093/femsre/fux020.
PMID: 28981745

Structural Insights into Outer Membrane Permeability of Acinetobacter baumannii
Structure. 2016 Feb 2;24(2):221-31. doi: 10.1016/j.str.2015.12.009. Epub 2016 Jan 21.
PMID: 26805524

Proteins causing membrane fouling in membrane bioreactors
Water Sci Technol. 2015;72(6):844-9. doi: 10.2166/wst.2015.282.
PMID: 26360742

Substrate specificity within a family of outer membrane carboxylate channels
PLoS Biol. 2012 Jan;10(1):e1001242. doi: 10.1371/journal.pbio.1001242. Epub 2012 Jan 17.
PMID: 22272184

Structure of a putative BenF-like porin from Pseudomonas fluorescens Pf-5 at 2.6 A resolution
Proteins. 2010 Nov 1;78(14):3056-62. doi: 10.1002/prot.22829.
PMID: 20737437

Structural insight into OprD substrate specificity
Nat Struct Mol Biol. 2007 Nov;14(11):1108-9. doi: 10.1038/nsmb1304. Epub 2007 Oct 21.
PMID: 17952093

Role of the novel OprD family of porins in nutrient uptake in Pseudomonas aeruginosa
J Bacteriol. 2006 Jan;188(1):45-54. doi: 10.1128/JB.188.1.45-54.2006.
PMID: 16352820

Analysis of the Pseudomonas aeruginosa oprD gene from clinical and environmental isolates
Environ Microbiol. 2002 Dec;4(12):872-82. doi: 10.1046/j.1462-2920.2002.00281.x.
PMID: 12534469

Function of pseudomonas porins in uptake and efflux
Annu Rev Microbiol. 2002;56:17-38. doi: 10.1146/annurev.micro.56.012302.160310. Epub 2002 Jan 30.
PMID: 12142471

C-terminal region of Pseudomonas aeruginosa outer membrane porin OprD modulates susceptibility to meropenem
Antimicrob Agents Chemother. 2001 Jun;45(6):1780-7. doi: 10.1128/AAC.45.6.1780-1787.2001.
PMID: 11353625

Amino acid-mediated induction of the basic amino acid-specific outer membrane porin OprD from Pseudomonas aeruginosa
J Bacteriol. 1999 Sep;181(17):5426-32. doi: 10.1128/JB.181.17.5426-5432.1999.
PMID: 10464217

Identification of the catalytic triad of the protein D2 protease in Pseudomonas aeruginosa
Biochem Biophys Res Commun. 1998 Jun 9;247(1):142-5. doi: 10.1006/bbrc.1998.8745.
PMID: 9636669

Proteins in this family with 3D-structure
View Entry
Description
Organism
Length
# strands
Glycine-glutamate dipeptide porin OpdP
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM14847 / LMG 12228 / 1C / PRS 101 / PAO1)
484
18
Outer membrane porin, OprD family
Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
420
18
Pyroglutatmate porin OpdO
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM14847 / LMG 12228 / 1C / PRS 101 / PAO1)
409
18
Probable porin
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM14847 / LMG 12228 / 1C / PRS 101 / PAO1)
431
18
Probable porin
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM14847 / LMG 12228 / 1C / PRS 101 / PAO1)
418
18
Probable porin
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM14847 / LMG 12228 / 1C / PRS 101 / PAO1)
416
18
Probable porin
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM14847 / LMG 12228 / 1C / PRS 101 / PAO1)
416
18
Vanillate porin OpdK
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM14847 / LMG 12228 / 1C / PRS 101 / PAO1)
417
18
Cis-aconitate porin OpdH
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM14847 / LMG 12228 / 1C / PRS 101 / PAO1)
427
18
Probable porin
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM14847 / LMG 12228 / 1C / PRS 101 / PAO1)
416
18
Histidine porin OpdC
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM14847 / LMG 12228 / 1C / PRS 101 / PAO1)
444
18
Probable porin
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM14847 / LMG 12228 / 1C / PRS 101 / PAO1)
421
18
Probable porin
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM14847 / LMG 12228 / 1C / PRS 101 / PAO1)
421
18
Anaerobically-induced outer membrane porin OprE
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM14847 / LMG 12228 / 1C / PRS 101 / PAO1)
460
18
Bacteriophage adsorption protein A
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM14847 / LMG 12228 / 1C / PRS 101 / PAO1)
443
18
Benzoate transport porin BenP
Acinetobacter baumannii
418
18
OprD family porin
Acinetobacter baumannii
426
18


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