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The Outer membrane protein transport protein (OMPP1/FadL/TodX) Family [Function: Specific diffusion channels] Seed alignment | Full alignment | Pfam page | Pfam Wiki page | TC-DB page

This family includes several distantly related outer membrane proteins, sequenced from different bacterial species. The family is named after the Escherichia coli FadL protein which functions in transporting long chain fatty acids across the outer membrane. Other members (such as XylN and TodX) are implicated in the transport of aromatic compounds such as toluene, m-xylene and its analogs. All members seem to function as monomers. The 3D structure of FadL at high resolution shows that the protein forms a monomeric ß-barrel composed of 14 ß strands, that is occluded by a central hatch domain. The hatch domain is composed of residues in the N-terminus in a similar manner with that found in the TonB-dependent receptors. The hydrophobic compounds probably bind to multiple sites in the channel and use a transport mechanism that involves a spontaneous conformational change in the hatch region.


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Literature references


Novel Bacterial Surface Display System Based on the Escherichia coli Protein MipA
J Microbiol Biotechnol. 2020 Jul 28;30(7):1097-1103. doi: 10.4014/jmb.2001.01053.
PMID: 32325544

Antagonistic Pleiotropy in the Bifunctional Surface Protein FadL (OmpP1) during Adaptation of Haemophilus influenzae to Chronic Lung Infection Associated with Chronic Obstructive Pulmonary Disease
mBio. 2018 Sep 25;9(5):e01176-18. doi: 10.1128/mBio.01176-18.
PMID: 30254117

Production of (Z)-11-(heptanoyloxy)undec-9-enoic acid from ricinoleic acid by utilizing crude glycerol as sole carbon source in engineered Escherichia coli expressing BVMO-ADH-FadL
Enzyme Microb Technol. 2018 Dec;119:45-51. doi: 10.1016/j.enzmictec.2018.09.001. Epub 2018 Sep 5.
PMID: 30243386

Intracellular transformation rates of fatty acids are influenced by expression of the fatty acid transporter FadL in Escherichia coli cell membrane
J Biotechnol. 2018 Sep 10;281:161-167. doi: 10.1016/j.jbiotec.2018.07.019. Epub 2018 Jul 21.
PMID: 30016739

Identification of ethanol tolerant outer membrane proteome reveals OmpC-dependent mechanism in a manner of EnvZ/OmpR regulation in Escherichia coli
J Proteomics. 2018 May 15;179:92-99. doi: 10.1016/j.jprot.2018.03.005. Epub 2018 Mar 6.
PMID: 29518576

Improving Escherichia coli membrane integrity and fatty acid production by expression tuning of FadL and OmpF
Microb Cell Fact. 2017 Feb 28;16(1):38. doi: 10.1186/s12934-017-0650-8.
PMID: 28245829

Molecular variation of the nonribosomal peptide-polyketide siderophore yersiniabactin through biosynthetic and metabolic engineering
Biotechnol Bioeng. 2016 May;113(5):1067-74. doi: 10.1002/bit.25872. Epub 2015 Nov 20.
PMID: 26524346

Outer membrane proteomics of kanamycin-resistant Escherichia coli identified MipA as a novel antibiotic resistance-related protein
FEMS Microbiol Lett. 2015 Jun;362(11):fnv074. doi: 10.1093/femsle/fnv074. Epub 2015 May 3.
PMID: 25940639

Substrate uptake and subcellular compartmentation of anoxic cholesterol catabolism in Sterolibacterium denitrificans
J Biol Chem. 2015 Jan 9;290(2):1155-69. doi: 10.1074/jbc.M114.603779. Epub 2014 Nov 21.
PMID: 25418128

Identification of membrane proteins associated with phenylpropanoid tolerance and transport in Escherichia coli BL21
J Proteomics. 2015 Jan 15;113:15-28. doi: 10.1016/j.jprot.2014.09.012. Epub 2014 Sep 30.
PMID: 25277045

fadD deletion and fadL overexpression in Escherichia coli increase hydroxy long-chain fatty acid productivity
Appl Microbiol Biotechnol. 2014 Nov;98(21):8917-25. doi: 10.1007/s00253-014-5974-2. Epub 2014 Aug 14.
PMID: 25117545

Rhizobial homologs of the fatty acid transporter FadL facilitate perception of long-chain acyl-homoserine lactone signals
Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10702-7. doi: 10.1073/pnas.1404929111. Epub 2014 Jul 7.
PMID: 25002473

The outer surface lipoprotein VolA mediates utilization of exogenous lipids by Vibrio cholerae
mBio. 2013 May 14;4(3):e00305-13. doi: 10.1128/mBio.00305-13.
PMID: 23674613

Functional characterization of ExFadLO, an outer membrane protein required for exporting oxygenated long-chain fatty acids in Pseudomonas aeruginosa
Biochimie. 2013 Feb;95(2):290-8. doi: 10.1016/j.biochi.2012.09.032. Epub 2012 Oct 12.
PMID: 23069386

Crosstalk of Escherichia coli FadR with global regulators in expression of fatty acid transport genes
PLoS One. 2012;7(9):e46275. doi: 10.1371/journal.pone.0046275. Epub 2012 Sep 28.
PMID: 23029459

Screening of the Salmonella paratyphi A CMCC 50973 strain outer membrane proteins for the identification of potential vaccine targets
Mol Med Rep. 2012 Jan;5(1):78-83. doi: 10.3892/mmr.2011.587. Epub 2011 Sep 13.
PMID: 21922141

Outer membrane proteome and its regulation networks in response to glucose concentration changes in Escherichia coli
Mol Biosyst. 2011 Nov;7(11):3087-93. doi: 10.1039/c1mb05193h. Epub 2011 Aug 18.
PMID: 21850335

Ligand-gated diffusion across the bacterial outer membrane
Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10121-6. doi: 10.1073/pnas.1018532108. Epub 2011 May 18.
PMID: 21593406

Transmembrane passage of hydrophobic compounds through a protein channel wall
Nature. 2009 Mar 19;458(7236):367-70. doi: 10.1038/nature07678. Epub 2009 Feb 1.
PMID: 19182779

Outer-membrane transport of aromatic hydrocarbons as a first step in biodegradation
Proc Natl Acad Sci U S A. 2008 Jun 24;105(25):8601-6. doi: 10.1073/pnas.0801264105. Epub 2008 Jun 16.
PMID: 18559855

The FadL family: unusual transporters for unusual substrates
Curr Opin Struct Biol. 2005 Aug;15(4):401-7. doi: 10.1016/j.sbi.2005.06.003.
PMID: 16005205

Bacterial long chain fatty acid transport: gateway to a fatty acid-responsive signaling system
J Biol Chem. 2004 Nov 26;279(48):49563-6. doi: 10.1074/jbc.R400026200. Epub 2004 Aug 30.
PMID: 15347640

Crystal structure of the long-chain fatty acid transporter FadL
Science. 2004 Jun 4;304(5676):1506-9. doi: 10.1126/science.1097524.
PMID: 15178802

The TOL plasmid pWW0 xylN gene product from Pseudomonas putida is involved in m-xylene uptake
J Bacteriol. 2001 Nov;183(22):6662-6. doi: 10.1128/JB.183.22.6662-6666.2001.
PMID: 11673437

Identification of a membrane protein and a truncated LysR-type regulator associated with the toluene degradation pathway in Pseudomonas putida F1
Mol Gen Genet. 1995 Mar 10;246(5):570-9. doi: 10.1007/BF00298963.
PMID: 7535376

Proteins in this family with 3D-structure
View Entry
Description
Organism
Length
# strands
TbuX
Ralstonia pickettii (Burkholderia pickettii)
458
14
Membrane protein involved in aromatic hydrocarbon degradation
Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1)
453
14
Outer membrane protein MtrB
Escherichia coli (strain K12)
446
14
Probable outer membrane protein
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM14847 / LMG 12228 / 1C / PRS 101 / PAO1)
463
14
TodX
Pseudomonas putida (Arthrobacter siderocapsulatus)
453
14


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