Please paste one or more FASTA-formatted sequences in the textarea below:



Select mode: MSA-version (1 sequence)   Single-sequence version (up to 5000 sequences)

Use: Original model   "dipeptides" model

Run signal-peptide prediction using PRED-TAT:

Use library of characteristic pHMMs for β-barrel regions derived from OMPdb in prediction:

Prior information (constrained prediction):

Enter your email address to get notification of results:



Note: If you wish to perform a batch submission of more than 5000 sequences, please contact kostas.tsirigosgmail.com directly.



- If you find PRED-TMBB2 useful in your research, please consider citing the main reference that describes this work:
PRED-TMBB2: Improved topology prediction and detection of beta-barrel outer membrane proteins
Konstantinos D. Tsirigos, Arne Elofsson and Pantelis G. Bagos
PMID: 27587687

- If you further use the new ("dipeptides") model, please also cite:
Extending Hidden Markov Models to allow conditioning on previous observations
Ioannis A. Tamposis, Margarita C. Theodoropoulou, Konstantinos D. Tsirigos and Pantelis G. Bagos
under review in Bioinformatics


Download the training dataset here
Download the discrimination dataset here