Please paste one or more FASTA-formatted sequences in the textarea below:



Select mode: MSA-version (1 sequence)   Single-sequence version (up to 5000 sequences)

Run signal-peptide prediction using PRED-TAT:

Use library of characteristic pHMMs for β-barrel regions derived from OMPdb in prediction:

Prior information (constrained prediction):

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Note: If you wish to perform a batch submission of more than 5000 sequences, please contact kostas.tsirigosgmail.com directly.



If you find PRED-TMBB2 useful in your research, please consider citing the reference that describes this work:

PRED-TMBB2: Improved topology prediction and detection of beta-barrel outer membrane proteins
Konstantinos D. Tsirigos, Arne Elofsson and Pantelis G. Bagos
(ECCB 2016 paper proceedings) / PMID: 27587687


Download the training dataset here
Download the discrimination dataset here